Direct neutralization of alkaline-denatured plasmid DNA in sequencing protocol by the sequencing reagent itself
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Direct neutralization of alkaline-denatured plasmid DNA in sequencing protocol by the sequencing reagent itself.
Denaturation of the double stranded DNA is required before sequencing by the dideoxy chain termination method (1). Good results are routinely obtained with the alkaline-denaturation method, which involves incubation of the DNA in the presence of 0.2 M NaOH for a few minutes at room or higher temperatures (2,3). The alkaline mixture is then neutralized by the addition of sodium acetate (0.3 M), ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1994
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/22.22.4844